Treatmeanntation of methyl – phenanthrene by bioremediation of soil polluted by fuel oil

Abstract

In this work, the ex situ natural bioremediation of soil polluted by heavy fuel oil from an oil refinery, was conducted on period about of six months. Phenanthrene and methyl-phenanthrenes are most Aromatic pollutants originating specially from fuel oil. Phenanthrene is usually degraded faster than methyl-phenanthrenes under different environmental conditions. Here, we noted a preferential and accelerated biodegradation of methyl –phenanthrenes versus phenanthrenes in soil contaminated by fuel oil. The polluted soil is mixed microbial consortia isolated from crude oil contaminated soil and treated by bio-surfactants and nutritive substances for biostimulation. In the present investigation, the ex situ natural bioremediation of soil for  a steady increase in the relative abundances of phenanthrene  compared  to methyl phenanthrene  was observed by gas chromatography –mass spectrometry the increase was the highest for trimethyl phenanthrenes.